Extraction Optimization of Pigeon Pea Seed Protein and Yield Using Response Surface Methodology

Pigeon Pea (Casanus Cajan L.) is one of the local foods in South Sulawesi. pea seeds contain protein that body can absorb. This study aims to determine optimum operating conditions for extracting pigeon on and yield using Ultrasonic-Assisted Extraction (UAE). Response Surface Methodology (RSM) with Box-Behnken Design (BBD) was used by considering three variables, time (30, 40, 50 min), temperature (40, 50, 60oC), methanol concentration (50, 70, 90%). The experiment results show ranges from 8.85663% 31.4766% 46.1538 mg/l 15584.6 mg/l. variables were computed create contour plot graphs. sonication extraction a 44.760 minutes, 60oC, 90% concentration.